Fecal microbiota of three bactrian camels (Camelus ferus and Camelus bactrianus) in China by high throughput sequencing of the V3-V4 region of the 16S rRNA gene
1 Xinjiang Lop Nor Wild Camel National Nature Reserve, Urumqi 830011, China; 2 Animal Science Department, Xinjiang Agricultural University, Urumqi 830052, China; 3 Key Laboratory of Biogeography and Bioresources in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese
Academy of Sciences, Urumqi 830011, China; 4 The People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
Fecal microbiota of three bactrian camels (Camelus ferus and Camelus bactrianus) in China by high throughput sequencing of the V3-V4 region of the 16S rRNA gene
1 Xinjiang Lop Nor Wild Camel National Nature Reserve, Urumqi 830011, China; 2 Animal Science Department, Xinjiang Agricultural University, Urumqi 830052, China; 3 Key Laboratory of Biogeography and Bioresources in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese
Academy of Sciences, Urumqi 830011, China; 4 The People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
摘要 This study aimed to reveal the microbial diversity in the fecal samples of bactrian camels using
the 16S rRNA sequencing analysis on the Illumina MiSeq platform. Three fecal samples were collected
from two geographical regions in China. Operational taxonomic unit (OTU) clustering was performed by
identifying an OTU at 97% sequence identity. The alpha and beta diversities were applied to estimate the
differences in microbial diversity among the three fecal samples. Totally, 4409, 3151 and 4075 OTUs in the
fecal samples were identified in the Lop Nor wild camel (Camelus ferus), the domestic camel (C. bactrianus)
and Dunhuang wild camel (C. ferus), respectively. The majority of bactreria were affiliated with phylum
Firmicutes and Bacteroidetes in the three samples. The wild camels had higher gastrointestinal tract
microbial diversity than the domestic one, while the microbial composition of the Lop Nor wild camel
shared higher similarity with domestic camel at the genus and family levels than that of the Dunhuang
wild camel did. Our results may provide a theoretical basis for assessing their health conditions and may
thus be useful for protecting the critically endangered species of C. ferus.
Abstract：
This study aimed to reveal the microbial diversity in the fecal samples of bactrian camels using
the 16S rRNA sequencing analysis on the Illumina MiSeq platform. Three fecal samples were collected
from two geographical regions in China. Operational taxonomic unit (OTU) clustering was performed by
identifying an OTU at 97% sequence identity. The alpha and beta diversities were applied to estimate the
differences in microbial diversity among the three fecal samples. Totally, 4409, 3151 and 4075 OTUs in the
fecal samples were identified in the Lop Nor wild camel (Camelus ferus), the domestic camel (C. bactrianus)
and Dunhuang wild camel (C. ferus), respectively. The majority of bactreria were affiliated with phylum
Firmicutes and Bacteroidetes in the three samples. The wild camels had higher gastrointestinal tract
microbial diversity than the domestic one, while the microbial composition of the Lop Nor wild camel
shared higher similarity with domestic camel at the genus and family levels than that of the Dunhuang
wild camel did. Our results may provide a theoretical basis for assessing their health conditions and may
thus be useful for protecting the critically endangered species of C. ferus.
The Xinjiang Lop Nur Wild Camels National Reserve Comprehensive Scientific
Research Projects by The Environmental Protection Agency of China (20100228).
通讯作者: QI Aladaer
E-mail: aqicas@foxmail.com
引用本文:
YUAN Lei,QI Aladaer,CHENG Yun等. Fecal microbiota of three bactrian camels (Camelus ferus and Camelus bactrianus) in China by high throughput sequencing of the V3-V4 region of the 16S rRNA gene[J]. Journal of Arid Land, 2017, 9(1): 153-159.
YUAN Lei,QI Aladaer,CHENG Yun et al. Fecal microbiota of three bactrian camels (Camelus ferus and Camelus bactrianus) in China by high throughput sequencing of the V3-V4 region of the 16S rRNA gene[J]. Journal of Arid Land, 2017, 9(1): 153-159.
2017, Vol. 9 
