Abstract: In this work, a new type of three-dimensional isolation system with high static and low dynamic stiffness (HSLD) characteristics is proposed. The HSLD isolation system is composed of the inclined rubber bearings with large load capacity providing positive stiffness and the devices providing negative stiffness. In the static stage, the inclined rubber bearing is used to provide enough stiffness to control the static displacement, while in the dynamic stage the positive and negative stiffness devices are combined to achieve small isolation stiffness and reduce the acceleration response, resolving the conflict of low isolation stiffness and high bearing capacity of three-dimensional isolation support. Further analysis on the deformation mechanism of inclined rubber bearing and negative stiffness device was performed to obtain the mechanical models of two devices and the important parameters. The static tests of inclined rubber bearing and negative stiffness device were conducted, and the theoretical mechanical models of two types of devices were established. By analyzing the structural dynamic response under different levels of earthquakes, it is found that the HSLD isolation system can meet the design requirements of seismic performance at different levels, and the static displacement and seismic response of the entire structure can be effectively control.