Abstract：Bio-grouting is a relatively new and innovative ground reinforcement method. However, it is hard to make a systematic and comprehensive research on the influencing factors by experiments because it is a complex physical and biochemical process. The 1D finite-element simulation method for the bio-grouting process in sand column is used to analyze the impact of several factors that influence on the distribution of calcium carbonate precipitation along the sand column and the precipitation rate after reaction. In this paper, the simulation results and the experimental ones are compared to validate the 1D finite-element model. And then the factors affecting the bio-grouting process are analyzed by simulation results, including the initial enzyme activity, the grouting rate, the concentration of nutrient solution and the no-flow reaction time. The simulation results indicate that the initial enzyme activity and grouting rate have great influence on the homogeneity of the distribution of calcium carbonate. Appropriate nutrient concentration can contribute to more calcium carbonate precipitation and it will take a certain amount of time to finish the total precipitation.
