The high ductile fiber concrete (HDFC) have good deformation capacity and fatigue resistance capacity, and can be used in big concrete engineering structure bearing the repeated loads. In order to study the fatigue properties, 54 specimens were made to conduct tension and compression fatigue test under constant amplitude loading. After testing the extreme value of static tensile strength, the fatigue life parameters of HDFC were studied by the stress level of 0.7, 0.8, 0.9, and the frequency of 10Hz, 20Hz and 30Hz. According to the fatigue cumulative damage theory, the law of fatigue strength and deformation of concrete is analyzed. The results show that the fatigue load is consistent with the Weibull two-parameter distribution, and the corresponding p-S-N equation is given. The research results can provide reference for the analysis of the seismic design of concrete structure and the vibration of bridge.