Abstract: In this work, glued and laminated bamboo (glubam) and SPF (spruce-pine-fir) wood were combined to form the cross-laminated bamboo and timber (CLBT) columns and cross-laminated timber (CLT) columns for experimental study. The laws of vertical strain, transverse strain, lateral deflection and ultimate bearing capacity varying with the slenderness ratio were analyzed. The cross-laminated bamboo and timber and cross-laminated timber columns were compared, and then the changes of the ultimate bearing capacity of the columns were analyzed. Furthermore, the failure modes and mechanism of cross-laminated bamboo and timber columns and cross. laminated timber columns were discussed. Finally, by referring to the domestic and foreign design codes of wooden structure, the bearing capacity of the specimen was calculated based on the improved bearing capacity formula of CLBT and compared with the test value. The results show that the ultimate bearing capacity of the CLBT column is 40.49% ~ 61.01% higher than that of the CLT column; the lateral stiffness is significantly improved; the peak longitudinal strain is increased by 30.45%~56.11%; the experimental values of cross-laminated bamboo and timber and cross-laminated timber are in good agreement with the theoretical values calculated by referring to the foreign design codes of timber structure and the improved bearing capacity formula of CLBT; the theoretical values calculated by CLBT and CLT tend to be more conservative with the increase of the slenderness ratio of the specimen.