In this study, an experimental investigation was carried out for the shear behavior of ultra-high performance concrete (UHPC) beams with stirrups through flexural tests under a point loading, where tension strain-hardening and multiple cracking characteristics can be observed in UHPC. Seven UHPC beams were tested in the shear test with the test variables being the shear-span ratios, longitudinal reinforcement ratio, stirrup content and steel fiber volume, etc. The experimental results show the shear failure modes of UHPC beams with stirrups shall be shear failure after bending yield and shear-compression failure. Multiple diagonal cracks can be seen on the surfaces of UHPC beams. With the increase of fiber content, the initial cracking strength may increase significantly and the ultimate shear strength can also be improved. The use of sufficient stirrups and steel fibers may meet the strength and ductility requirements of the UHPC beams. If the stirrup content is increased, more dense and short inclined cracks may appear at the web of UHPC beam. In this work, the computational model is proposed for the theoretical shear capacity of UHPC beams, which include the contributions of the concrete in compressed zone, the steel fiber at diagonal crack, the stirrups and the dowel action. It is found that the calculated results based on the model may agree very well with the measured ones.